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. 2011 Jan 27;17:291–299.

Figure 3.

Figure 3

The enhanced specific lentiviral vector combination induces high HSVtk protein expression in HLECs. After transfection with LTKCRE, PGFPTK, and the enhanced specific lentiviral vector combination, the HLECs (A-C) and RPECs (D-F) were observed under a fluorescence microscope and FACS at 72 h. Cells infected with LTKCRE did not express EGFP (A, D). The transduction efficiency of PGFPTK measured by FACS was 97.43% and 92.15% in HLECs and RPECs, respectively (B, E). Meanwhile the transduction efficiency of the enhanced specific lentiviral vector combination in HLECs and RPECs was 6.31% and 86.46%, respectively. Cre, EGFP, and HSV-tk protein expressions in HLECs were analyzed by western blot for cells infected by different lentiviral vectors (G).