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pRB is O-GlcNAc modified in vivo. In duplicate, pRB from pre-cleared lysates of HeLa S3 cells was immunopurified and separated by SDS–PAGE. Following transfer, pRB was detected by Western blotting and the presence of the O-GlcNAc modification by lectin blotting with O-GlcNAc specific sWGA coupled to HRP
