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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Dec 6;506(2):157–164. doi: 10.1016/j.abb.2010.12.002

Figure 1. HPLC Analysis of the ADH3-catalyzed Oxidation of N-Benzoylethanolamine.

Figure 1

Reaction conditions were as follows: 100 mM sodium pyrophosphate pH 9.5, 2.5 mM NAD+, 6.0 mM N-benzoylethanolamine, and 2.4 mg/ml ADH3. HPLC analysis was performed after 36 hr of reaction time. The peak with a retention time of 3.918 min was derived from NAD+ that formed during prolonged incubation at high pH. A peak with the same retention time was also observed in the control sample containing only NAD+ and buffer.