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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Dec 6;506(2):157–164. doi: 10.1016/j.abb.2010.12.002

Figure 3. GC-MS Analysis of the ADH3-generated Cinnamyl Aldehyde.

Figure 3

Cinnamyl aldehyde was first generated enzymatically by treating cinnamyl alcohol with ADH3 as described in the legend to Figure 2. Cinnamyl aldehyde was converted to the perfluorobenzyl oxime (O-PFB-oxime) with PFBHA and analyzed by GC-MS. The resulting cinnamyl aldehyde O-PFB-oxime exists as the E- and Z-isomers, which have different retention times in the gas chromatograph (upper panel), but exhibit identical fragmentation patterns from the mass spectrometer (lower panel). The structure and fragmentation pattern of cinnamaldehyde O-perfluorobenzyl oxime are shown in the inset to the lower panel.