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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Dec 6;506(2):157–164. doi: 10.1016/j.abb.2010.12.002

Table 1.

Kinetic Constants for the ADH3-Mediated Oxidation of NAE Substrates with Acyl Chains Containing 2-12 Carbon Atoms.

Substrate graphic file with name nihms262690t1.jpg KM (mM) VMAX (μmol/min/mg) VMAX/KM (sec−1 M−1)
N-Acetylethanolamine R = CH3 (4.5 ± 0.4) × 102 1.9 ± 0.059 2.7
N-Propionylethanolamine R = CH3CH2 79 ± 3.8 1.9 ± 0.026 15
N-Butyrylethanolamine R = CH3(CH2)2 46 ± 5.4 1.7 ± 0.052 23
N-Hexanoylethanolamine R = CH3(CH2)4 9.5 ± 0.36 1.6 ± 0.018 110
N-Octanoylethanolamine R = CH3(CH2)6 5.8 ± 0.38 1.5 ± 0.028 160
N-Decanoylethanolamine R = CH3(CH2)8 0.32 ± 0.029 0.57 ± 0.024 1.1 × 103
N-Lauroylethanolamine R = CH3(CH2)10 0.033 ± 0.0061 0.14 ± 0.0088 2.7 × 103
1-Octanol CH3-(CH2)6-CH2-OH 0.050 ± 0.0130 2.5 ± 0.16 3.2 × 104
Cinnamyl alcohol C6H6HC=CHCH2OH 0.035 ± 0.0033 4.0 ± 0.058 7.2 × 104