. Author manuscript; available in PMC: 2012 Feb 15.

Published in final edited form as: Arch Biochem Biophys. 2010 Dec 6;506(2):157–164. doi: 10.1016/j.abb.2010.12.002

Table 2.

Kinetic Constants for the ADH3-Mediated Oxidation of NAE Substrates with Acyl Chains Containing an Benzyl Ring.

Substrate		K_M (mM)	V_Max (μmol/min/mg)	V_Max/K_M (sec⁻¹ M⁻¹)
N-Benzoylethanolamine	R = C₆H₆	5.2 ± 0.52	0.36 ± 0.0098	44
N-Phenylacetylethanolamine	R = C₆H₅CH₂	3.9 ± 0.24	1.2 ± 0.026	200
N-Benzyloxycarbonylethanolamine	R = C₆H₅CH₂O	2.3 ± 0.14	0.81 ± 0.014	220
N-(2-Phenoxyacetyl)ethanolamine	R = C₆H₅OCH₂	6.3 ± 0.36	0.25 ± 0.0042	25
Cinnamyl alcohol	C₆ H₆HC=CHCH₂	0.035 ± 0.0033	4.0 ± 0.058	7.2 × 10⁴