. Author manuscript; available in PMC: 2012 Feb 15.

Published in final edited form as: Arch Biochem Biophys. 2010 Dec 6;506(2):157–164. doi: 10.1016/j.abb.2010.12.002

Table 3.

Comparison of the K_M Values to the Calculated Free Energy of Binding for Selected NAEs and Cinnamyl Alcohol.

Substrate	K_M (mM)	Calculated Free Energy of Binding^a (kcal/mol)
N-Acetylethanolamine	(4.5 ± 0.4) × 10²	−3.72
N-Propionylethanolamine	79 ± 3.8	−4.14
N-Butyrlethanolamine	46 ± 5.4	−4.22
N-Hexanoylethanolamine	9.5 ± 0.36	−4.34
N-Octanoylethanolamine	5.8 ± 0.38	−4.31
N-Decanoylethanolamine	0.32 ± 0.029	−4.34
N-Lauroylethanolamine	0.033 ± 0.0061	−4.06
N-Benzoylethanolamine	5.2 ± 0.52	−4.58
Cinnamyl alcohol	0.035 ± 0.0033	−4.68

Binding energies values were calculated using Autodock 4.0, and have a standard error of about ± 2.5 kcal/mol [39].