Figure 4.

Use of NMR to assess CHOBIMALT micelle size and concentration-dependent properties. A. Overlay of decays observed using an STE-BP sequence to measure the translational diffusion coefficient of CHOBIMALT at 1.0% (10 mM, red) and at 0.1% (1 mM, black). B. Comparison of 1-D ¹H NMR spectra acquired at decreasing CHOBIMALT concentrations: (from bottom to top) 10mM (32 scans), 1 mM (512 scans), 100 μM (4096 scans), 1 μM (4096 scans). Asterisks indicate resonances appearing at low concentration from trace contaminants. C. Comparison of 1D ¹H NMR spectra of detergent-like cholesterol analogs. The 15.8 mM CHAPSO sample represents a rapid exchange mixture between monomeric CHAPSO (estimated to be 8 mM based on its CMC) and micellar CHAPSO. Note that CHAPSO, like other bile salt-derived detergents, is believed to form very small micelles. All NMR spectra were acquired at 500 MHz at 20°C.