Table 2.

Mean ED₅₀ values and potency ratios with 95% confidence intervals (CI) for the rate-decreasing effects of pregnanolone, flunitrazepam, midazolam, morphine, ketamine, and pentobarbital before, during, and after daily pregnanolone treatment. Dose-effect curves were determined 1 and 25 hours after the previous pregnanolone dose.

	ED50 (mg/kg)		Potency Ratioa
	Mean	95% CI	Mean	95% CI
Pregnanolone
Before	12.95	10.68-15.21
During
1 hour	12.01	7.68-16.35	0.97	0.56-1.38
25 hours	11.85	8.73-14.98	0.94	0.67-1.20
After	7.94	5.49-10.39	0.63*	0.43-0.82
Flunitrazepam
Before	1.72	1.03-2.40
During
1 hour	0.58	0.08-1.08	0.49*	0.17-0.82
25 hours	0.55	0.26-0.84	0.33*	0.19-0.47
After	1.63	0.27-3.00	0.92	0.43-1.40
Midazolam
Before	0.85	0.44-1.26
During
1 hour	0.85	0.48-1.22	1.23	0.58-1.88
25 hours	1.45	0.86-2.04	1.96*	1.09-2.82
After	0.37	0.20-0.56	0.55*	0.22-0.88
Pentobarbital
Before	6.73	6.05-7.41
During
1 hour	9.92	7.53-12.31	1.48*	1.14-1.83
25 hours	9.48	5.84-13.11	1.42	0.84-2.00
After	8.79	6.7-10.89	1.32	1.00-1.64
Ketamine
Before	1.62	1.27-1.97
During
1 hour	7.43	4.65-10.22	4.90*	2.43-7.37
25 hours	4.34	2.69-5.99	2.88*	1.48-4.28
After	1.67	1.24-2.11	1.10	0.70-1.50
Morphine
Before	3.05	2.04-4.06
During
1 hour	3.11	0.50-5.72	1.24	-0.05-2.53
25 hours	2.16	1.50-2.83	0.85	0.39-1.30
After	2.04	1.36-2.72	0.79	0.31-1.28

^aPotency ratio relative to ED₅₀ values before treatment.

^*Indicate significant differences from before treatment.