Figure 3.

Average number of nuclear segments (lobulation index), calculated by the “Drittelregel” for individuals with zero to three functional (wild type) LBR-genes. The bar chart is based on the analysis of 26 individuals. 50 neutrophil nuclei were analyzed each. The error bar refers to the standard error of the mean. Two individuals had no functional (wild type) LBR-gene. One was homozygous for the splice mutation IVS12-5-10del (S), for the other homozygous the mutation was not identified thus far, but is probably regulatory ((R); detailed description in the materials and methods section). Nine individuals had one functional (wild type) LBR copy. Four of them were heterozygous for null mutations (N), five were heterozygous for a splice mutation (S). Twelve individuals with two functional (wild type) LBR copies were analyzed as controls. Three individuals had a triplication of the LBR-gene.