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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Nat Neurosci. 2010 Dec 8;14(2):200–207. doi: 10.1038/nn.2728

Figure 5.

Figure 5

Persistent firing induced by antidromic stimulation and inter-cellular signaling. (a) Diagram depicting recording setup (axon red, dendrites blue). For antidromic stimulation, the stimulating electrode was placed in the molecular layer of the dentate gyrus (DG) to activate only axonal projections. (b) Antidromic stimulation of the axon during simultaneous somatic hyperpolarization also evoked persistent firing (in the presence of glutamate and GABA receptor blockers; see online Methods). The inset shows failed spikes during antidromic stimulation. The number of stimuli was increased by two during each successive stimulus until persistent firing occurred. Persistent firing was reliably induced in this way (n = 5). (c) Illustration of paired recording set-up: step current injections were delivered to cell 1 only. Interneurons near the SR-SLM border and within the SR were targeted. (d) Persistent firing was induced in the unstimulated cell and occurred before persistent firing was induced in the stimulated cell. No electrical coupling was observed between the pairs. In total, 19 Htr5b-EGFP-positive pairs were studied with 3 showing this type of inter-cellular induction of persistent firing.