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Effects of NONOates and S-nitrosothiols on proMMP-9 activation. Purified proMMP9 (final concentration 0.2 µg/mL) was exposed to the indicated concentrations (µM) of NONOates or S-nitrosothiols, and gelatinase activity was monitored using DQ-Gelatin by fluorescence (485 Ex/530 Em). The rate of fluorescence increase was expressed relative to untreated controls (mean ± S.E.M; n = 4). For comparison, proMMP-9 activation by pCMB (200 µM) served as a positive control.
