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. Author manuscript; available in PMC: 2011 Jul 27.
Published in final edited form as: Nature. 2011 Jan 19;469(7331):539–542. doi: 10.1038/nature09639

Figure 4. Knockdown of PBRM1 expression in RCC cell lines.

Figure 4

(A) Verification of PBRM1 knockdown by western blotting. (B)Silencing PBRM1 increased the proliferation of ACHN and 786-O with wild type PBRM1, but not A704 with a homozygous PBRM1 truncating mutation. Data represent means of triplicate experiments with standard deviation, p<0.01. (C) Knockdown of PBRM1 enhanced colony formation in SN12C cells. Data represent means of triplicate experiments with standard deviation, p<0.01. (D) Knockdown of PBRM1 enhanced cell migration in 786-O, SN12C and TK10 cells. Data represent means of triplicate experiments with standard deviation, p<0.01. (E) Gene sets that are most significantly deregulated following PBRM1 knockdown in three RCC cell lines using curated gene sets obtained from MSigDB (http://www.broadinstitute.org/gsea/msigdb/) and additional curated gene sets obtained from the PGSEA package (see Supplemental Material for details).