Fig. 6.

a The most probable positions of the Arg side chain centers of charge in the single Arg-containing polyleucine helices (colored circles) mapped onto the atomistic PMF for Gdm translocation across a POPC bilayer (solid black curve). b Free energies of insertion, ΔG _app, from translocon-mediated insertion experiments on single-Arg-containing TM helices (Hessa et al. 2005b). The colored circles correspond to the simulated systems; the circles for R7 and R13 were placed by linear interpolation between the two neighboring points (Color figure online)