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. Author manuscript; available in PMC: 2011 Jan 30.

Published in final edited form as: J Infect Dis. 2008 Jan 15;197(2):225–234. doi: 10.1086/524687

Analysis of the broad host range of recombinant phage 149::Km^r. A, Representative restriction map of the att-L proximal region of 149::Km^r. Genes are drawn to scale. Small, black arrows, positions of primers. Sizes of PCR and hybridizing restriction fragments are indicated in bp. B, Results of polymerase chain reaction analysis of genomic DNA from 149 donor strain RDN149, 149::Km^r donor strain EC516, recipient strain RDN156 (M28), and lysogenized strains EC749 and EC917, with primers OLEC178 and oliRN70 (top), oliRN69 and OLEC179 (middle), and oliRN111 and oliRN110 (bottom). M, 1-kb DNA ladder (Fermentas Life Sciences). C, Results of Southern blot analysis of HindIII-digested genomic DNA from 149::Km^r donor strain EC516, phage recipient strain RDN156 (M28) and corresponding lysogenized strains EC749 and EC917, phage recipient strain RDN116 (M94) and corresponding lysogenized strains EC727 and EC1083 with α-³²P labelled DNA probes specific to the ssa upstream fragment (ssaup), aphIII gene, and ssa downstream fragment (ssadw).

Inline graphic — Analysis of the broad host range of recombinant phage 149::Km^r. A, Representative restriction map of the att-L proximal region of 149::Km^r. Genes are drawn to scale. Small, black arrows, positions of primers. Sizes of PCR and hybridizing restriction fragments are indicated in bp. B, Results of polymerase chain reaction analysis of genomic DNA from 149 donor strain RDN149, 149::Km^r donor strain EC516, recipient strain RDN156 (M28), and lysogenized strains EC749 and EC917, with primers OLEC178 and oliRN70 (top), oliRN69 and OLEC179 (middle), and oliRN111 and oliRN110 (bottom). M, 1-kb DNA ladder (Fermentas Life Sciences). C, Results of Southern blot analysis of HindIII-digested genomic DNA from 149::Km^r donor strain EC516, phage recipient strain RDN156 (M28) and corresponding lysogenized strains EC749 and EC917, phage recipient strain RDN116 (M94) and corresponding lysogenized strains EC727 and EC1083 with α-³²P labelled DNA probes specific to the ssa upstream fragment (ssaup), aphIII gene, and ssa downstream fragment (ssadw).