Fig. 1.

Alphasatellite-mediated VIGS of the β-glucuronidase (GUS) gene in the N. tabacum T19 line after co-agroinoculation with TbCSV

(a) Diagram of the 2mDNA1 structure. 2mDNA1 contains two direct repeats of the TbCSV DNA1 and a multiple cloning site (MCS) downstream of Rep for subsequent insertion of a DNA fragment targeted for silencing (Huang et al., 2009); (b–e) VIGS of the GUS gene in N. tabacum T19 line leaf (b), stem (c, d), and root (e) co-infected with TbCSV and either 2mDNA1 vector or 2mDNA1-GUS; (f) Silencing efficiency in GUS-silenced or non-silenced (control) N. tabacum T19 plants as revealed by real-time RT-PCR at 30 dpi. Three samples were tested and error bars represent the standard deviation of the mean