Fig. 3.

Effect of NtEDS1 on N. tabacum cv. Xanthi resistance against TMV

(a) NtEDS1-silenced plant does not express any visible phenotype; (b) TMV induces abundant necrosis lesions in inoculated leaf of NtEDS1 non-silenced plants but few in NtEDS1-silenced plants; (c) TMV symptoms are displayed in the systemic leaves of NtEDS1-silenced plant but not on NtEDS1 non-silenced plant; (d) Northern blot analysis shows TMV accumulation in inoculated (I) and systemic (S) leaves at 5 dpi using TMV movement protein (MP) gene as a probe; (e) RT-PCR analysis shows the effect of NtEDS1-silencing on PR1 transcription