Fig. 2.
Attenuation of the ICAM-1 protein expression in the HUVEC cells by MPostC. (A) The intercellular adhesion molecules-1 (ICAM-1) expression in the HUVECs is compared between the morphine postconditioning (MPostC) groups and the control group after 6 hr anoxia. The numbers of viable cells was 1 × 105 and the cell viability was 92%. The groups were divided to the control group and the 0.3, 3, and 30 µM MPostC groups. The mean fluorescence index (MFI) from each group was recorded at 0, 1, 3, 6, 9, and 12 hr. The valus are the mean ± SD of 6 experiments. *P < 0.05. (B) Phenotypical graph of the HUVECs. Flow cytometry analysis was done to characterize the ICAM-1 expressions on the HUVECs. PE Mouse Anti-Human CD54 monoclonal antibody was used to detect the ICAM-1 expression. The isotype antibody was used as the negative control (bold). The values were measured at 6 hr reperfusion time.