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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Hepatology. 2011 Jan 10;53(2):661–672. doi: 10.1002/hep.24085

Figure 5.

Figure 5

MAdCAM-1 induced in normal liver supports adhesion of JY cells and PBL from PSC patients. Static adhesion assays showing adhesion of (A) α4β7 positive JY cells and (B) peripheral blood lymphocytes from PSC patients, to normal liver sections cut from precision cut liver slices, after 4 hours stimulation with rVAP-1 and MA. Data represent mean adhesion ± SEM from n=4 normal livers and from n=3 PSC PBL. (C and D) Binding was inhibited when MAdCAM-1 (P1; 1μg/ml) and α4β7 (ACT-1; 1μg/ml) were blocked. Data show percent adherent cells compared with control samples and when blocking was used data show the percent adherent cells compared to adhesion in samples where no antibody was used (No Ab). Isotype-matched control (IMC) used at 1μg/ml. Student's t-test *p<0.01, **p<0.01, ***p<0.001. (E) Representative immunostaining pictures showing adherent lymphocytes in rVAP-1 and MA stimulated normal liver tissue and non-adhering lymphocytes on tissues where MAdCAM-1 was blocked (P1). Images were acquired at original magnification of x600 and x400, respectively.