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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Hepatology. 2011 Jan 10;53(2):661–672. doi: 10.1002/hep.24085

Figure 6.

Figure 6

VAP-1/SSAO induces MAdCAM-1 expression in-vivo. Wild type mice (WT) and VAP-1 deficient mice expressing enzymatically active (VAP-1_EA) and inactive (VAP-1_EI) human VAP-1, were supplied with 0.4% (w/v) methylamine (MA+) in their drinking water for 14 days. Wild type mice without methylamine treatment were controls (WT_MA−). (A) Immunofluorescent staining of MAdCAM-1 in high endothelial venules (HEVs) (white arrows) of Peyer's patches (PPs) and mesenteric lymph nodes (MLN) and in-vivo expression of human VAP- 1 in the liver of transgenic mice. Isotype-matched control (IMC) antibody was used as a negative control. (B) MAdCAM-1 mRNA analysis using real-time PCR. Data represent MAdCAM-1 mRNA fold change (mean±SD) in PPs and MLN in mice (n=3 in each group) receiving methylamine compared with wild-type mice that had not received methylamine (WT_MA−; n=3). *p<0.04 by Student's t-test in EA_MA+ vs WT_MA− in both PPs and MLN and *p=0.03 by Student's t-test in EA_MA+ vs EI_MA+ in MLN.