Figure 1.

Anti-cancer effect of CM-1 on colorectal cancer cell lines. (A) Anti-cancer effect of CM-1 in vitro. CLY and HT-29 cells were treated with graded concentrations of CM-1 for 72 h, and then cell growth was determined by MTT assay. Experiments were performed in triplicate and IC₅₀ values were determined by non-linear regression with dose-response curve fitting. Anti-tumour effect of CM-1 on CLY (B) and HT-29 (C) xenografts. Nude mouse xenograft models of CLY and HT-29 were established and treated with CM-1. Tumour volume was measured and compared every 2 or 3 days. The initial days showing significant differences between mice treated with 20 µg·kg⁻¹ CM-1, those given 10 µg·kg⁻¹ and the control untreated group are indicated as (*) and (#), respectively (one-way analysis of variance with Tukey's post hoc test, P < 0.05).