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. 2011 Jan;162(2):349–364. doi: 10.1111/j.1476-5381.2010.01042.x

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British Journal of Pharmacology © 2011 The British Pharmacological Society

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Effects of CM-1 on CM-1 sensitive cell line HEK293-135a1. (A) Cell treatments. miR-135 (C), pri-miR-135a1 (D), APC mRNA (E) and β-catenin mRNA (F) levels were analysed in HEK293-135a1 cells by qRT-PCR analysis. Adenomatous polyposis coli (APC), phospho-β-catenin and total β-catenin protein levels (B) were analysed in HEK293-135a1 cells by Western blot analysis. miRNA, mRNA and protein expression were normalized against 5s rRNA, β-actin mRNA and β-actin protein levels respectively. Each experiment was performed in triplicate.