Figure 7.
(A) Pretreatment with 2′,4′-dichlorobenzamil (DCB; 2.5.10−6 mol·L−1; white bar) prevents the changes in the Fura-2 ratio evoked by sodium hydrosulphide (NaHS) (10−4 mol·L−1; arrow) in comparison with control crypts not pretreated with DCB (B). (C) Time-dependent control without administration of NaHS. Isolated crypts were loaded with Fura-2 (as indicated by the schematic); given is the emission at an excitation wavelength of 340 nm divided by the emission at an excitation wave length of 380 nm. Values are given as means (symbols) ± SEM (grey shaded area), n = 52–84. For statistics, see Table 4.