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. Author manuscript; available in PMC: 2011 Nov 23.
Published in final edited form as: Curr Biol. 2010 Nov 4;20(22):2040–2045. doi: 10.1016/j.cub.2010.10.024

Figure 3. Aster movement depends on dynein dependent pulling forces.

Figure 3

A) Structure of caged-Combretastatin 4A. B) EMTB-3GFP zebrafish embryos were incubated in caged-Combretastatin and subjected to UV-irradiation in defined region (marked in red). Within seconds, microtubules depolymerised selectively on the irradiated side. The remaining aster moved away from the irradiated region, arguing for pulling forces on asters. Full arrows mark the positions of centrosomes, hollow arrows on right mark their original positions. C) Injection of p150-CC1 blocks aster movement. Asters still grew out and broke down under cell cycle control, but lost their ability to move or orient centrosomes. (See also Fig. S3, movies S3 and S4)