Table 4.

Effects of human SERT coding variants on SERT expression and regulation

hSERT variants	Effect on SERT Activity (versus wild type)
	Basal	PKG- activation	P38 MAPK-activation	PKC-activation	Surface expression
hSERT-WT	100%	Stimulation	Stimulation	Inhibition	100%
Thr4Ala1	Higher	Insensitive	Insensitive	More-sensitive	Similar to WT
Gly56Ala1,3*#	Higher	Insensitive	Insensitive	More-sensitive	Similar to WT
Lys201Asn2	Higher	NT	NT	NT	Similar to WT
Glu215Lys1	Similar to WT	Insensitive	Insensitive	Similar to WT	Similar to WT
Leu255Met1	Similar to WT	Similar to WT	Similar to WT	Similar to WT	Similar to WT
Ser293Phe1	Higher	Similar to WT	Similar to WT	Similar to WT	Similar to WT
Pro339Leu1	Lower	Similar to WT	Similar to WT	Similar to WT	Lower
Leu362Met1	Higher	Similar to WT	Similar to WT	Similar to WT	Similar to WT
Ile425Val11, 4	Higher	Insensitive	NT	Similar to WT	Similar/higher to WT
Ile425Leu3	Higher	Less-sensitive	Less-sensitive	< = Less-sensitive	Higher
Phe465Leu3*	Higher	Less-sensitive	Less-sensitive	< = Less-sensitive	Higher
Leu550Val3	Higher	Less-sensitive	Less-sensitive	< = Less-sensitive	Higher
Lys605Asn1	Similar to WT	Insensitive	Insensitive	More-sensitive	Similar to WT
Pro621Ser1	Similar to WT	Insensitive	Insensitive	More-sensitive	Similar to WT

NT: Not tested; < = comparable or slightly reduced sensitivity in comparison with WT;

^*Less sensitive to PP2 inhibition with calyculin and fostriecin in comparison with WT;

^#exhibits elevated basal phosphorylation.

¹(Prasad et al., 2005)

²(Rasmussen et al., 2009);

³(Prasad et al., 2009)

⁴(Kilic et al., 2003; Zhang et al., 2007)