Fig. 2.

Effect of β-arrestin 2 on Th1 and Th2 cytokine levels following restraint stress. Both wild-type (WT) and β-arrestin 2-deficient (β-arrestin 2 KO) mice aged 6–8 weeks were subjected to a 12-hour physical restraint stress for 2 days. Splenic lymphocytes were treated with concanavalin A (5 μg/ml) for 24 h (IFN-γ and IL-2 detection) or 48 h (IL-4). Production of the Th1 cytokines IFN-γ (a) and IL-2 (b), and the Th2 cytokine IL-4 (c) was analyzed by ELISA. Means and SE were calculated from 5 mice/group. ∗ p < 0.01 vs. unstressed WT control, ∗∗ p < 0.01 vs. stressed WT mice.