Fig. 2.
Effect of BQ3020 on coimmunoprecipitation of D3 and ETB receptors in rat RPT cells. The cells were incubated with BQ3020 (10−8 M) for 30 min. Thereafter, the samples were immunoprecipitated with anti-ETB antibodies and immunoblotted with anti-D3 antibodies (∗ p < 0.05 vs. control, # p < 0.05 vs. WKY rats, n = 8, ANOVA, Duncan's test). One immunoblot (45 kDa) is depicted in the inset (lane 1 = negative control, lane 2 = positive control, lane 3 = vehicle-treated RPT cell of WKY rats, lane 4 = BQ3020- treated RPT cell of WKY rats, lane 5 = vehicle-treated RPT cell of SHRs, lane 6 = BQ3020-treated RPT cell of SHRs).