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. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: Cell Signal. 2010 Oct 19;23(2):380–388. doi: 10.1016/j.cellsig.2010.10.008

(A) RT-PCR of the time course of effects of PTH on representative genes affected by G protein minigenes in UMR-106 cells. The cells were transfected with pcDNA, G_s minigene, G_q minigene, or G₁₂ minigene, and then treated with 10nM PTH for 0 hr, 2 hr, 8 hr, 24 hr, or 48 hr. (B) The relative expression levels of genes were analyzed and normalized against the housekeeping gene Rpl13a. The bands shown in Figure 3A were scanned and quantified for the analysis.

(A) RT-PCR of the time course of effects of PTH on representative genes affected by G protein minigenes in UMR-106 cells. The cells were transfected with pcDNA, G_s minigene, G_q minigene, or G₁₂ minigene, and then treated with 10nM PTH for 0 hr, 2 hr, 8 hr, 24 hr, or 48 hr. (B) The relative expression levels of genes were analyzed and normalized against the housekeeping gene Rpl13a. The bands shown in Figure 3A were scanned and quantified for the analysis.