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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Cell Signal. 2010 Oct 19;23(2):380–388. doi: 10.1016/j.cellsig.2010.10.008

Table 2.

Genes regulated by PTH and antagonized by G protein minigenes

A. PTH-regulated genes antagonized only by Gs minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene + PTH vs EV + PTH G12 minigene + PTH vs EV + PTH
Ratio P value Ratio P value Ratio P value
Myc 0.1 0.018 0.6 0.392 0.8 0.358
Egf 0.4 0.011 1.2 0.226 1.6 0.109
Pdgfa 0.2 0.003 1.3 0.407 1.2 0.307
Igfbp3 0.3 0.026 0.7 0.435 0.8 0.089
Cyp19a1 0.3 0.019 1.8 0.070 0.7 0.195
Rbp1 0.5 0.041 1.0 0.986 1.7 0.742
Fasn 2.1 0.010 0.5 0.397 0.6 0.580
Flt1 4.5 0.039 0.7 0.134 0.9 0.849
Fgf1 4.2 0.010 0.9 0.135 0.9 0.072
B. PTH-regulated genes antagonized only by Gq minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene +PTH vs EV + PTH G12 minigene + PTH vs EV+PTH
Ratio P value Ratio P value Ratio P value
Tgfb1 1.4 0.267 0.2 0.006 1.3 0.274
Egr1 08 0.708 0.4 0.021 1.0 0.991
Igf1 0.6 0.211 0.2 0.008 1.0 0.961
Tnf* 0.7 0.600 0.5 0.004 2.6 0.259
Tnf** 0.8 0.123 0.2 0.013 0.9 0.849
Bmp3 1.2 0.895 2.2 0.008 1.0 0.961
Bmp7 0.9 0.870 3.3 0.026 1.4 0.464
C. PTH-regulated genes antagonized only by G12 minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene +PTH vs EV + PTH G12 minigene + PTH vs EV+PTH
Ratio P value Ratio P value Ratio P value
Mmp8 1.2 0.365 1.3 0.573 0.1 0.008
Gadd45a 0.8 0.492 0.7 0.346 0.3 0.022
Foxa2 0.6 0.027 0.6 0.024 2.0 0.012
D. PTH-regulated genes antagonized by either Gs or Gq minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene + PTH vs EV + PTH G12 minigene + PTH vs EV+PTH
Ratio P value Ratio P value Ratio P value
Hsf1 0.4 0.002 0.5 0.003 1.0 0.574
Fgf4 0.2 0.032 0.3 0.044 0.6 0.133
Cdh1 0.2 0.004 0.2 0.006 1.0 0.744
Mmp7 0.3 0.042 0.2 0.016 1.5 0.253
Col3a1 0.2 0.014 0.5 0.017 1.1 0.675
Fn1* 0.4 0.012 0.4 0.015 0.7 0.252
Fn1** 0.3 0.035 0.3 0.025 1.5 0.079
E. PTH-regulated genes antagonized by either G2 or G12 minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene +PTH vs EV + PTH G12 minigene + PTH vs EV+PTH
Ratio P value Ratio P value Ratio P value
Cebpb 0.1 0.044 1.0 0.883 0.3 0.010
Coll4a1 0.1 0.012 0.8 0.200 0.4 0.009
Itgav 5.4 0.012 0.9 0.495 8.9 0.004
F. PTH-regulated genes antagonized by either Gs Gq or G12 minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene +PTH vs EV + PTH G12 minigene + PTH vs EV+PTH
Ratio P value Ratio P value Ratio P value
Fos 0.04 0.0002 0.1 0.0001 0.1 0.0002
Birc3 5.1 0.0015 5.3 0.0014 12.9 0.0006
Vegfa* 5.1 0.0015 2.5 0.0047 6.9 0.0010
Vegfa** 4.5 0.0192 2.5 0.0194 4.1 0.0090
G. PTH-regulated genes antagonized by neither Gs, Gq nor G12 minigene
Gene Symbol Gs minigene + PTH vs EV+PTH Gq minigene +PTH vs EV + PTH G12 minigene + PTH vs EV+PTH
Ratio P value Ratio P value Ratio P value
Pmepa1 0.7 0.238 0.7 0.272 0.8 0.423
Hk2 0.8 0.038 0.7 0.083 1.1 0.103
*

on signaling pathway array;

**

on osteogenesis array