Scheme 2.
AChE kinetic mechanism that accommodates substrate inhibition and includes an accumulating tetrahedral intermediate in the deacylation stage of catalysis.
P = thiocholine product
Q = acetate product
F = acylenzyme intermediate
T = tetrahedral intermediate
AF = acylenzyme intermediate with substrate at peripheral site
AEA = Michaelis complex with substrate at peripheral site
AT = tetrahedral intermediate with substrate at peripheral site
KA = dissociation constant of AEA, AF and AT complexes