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. 2010 Jul 20;148(4):467–479. doi: 10.1093/jb/mvq080

Fig. 4.

Fig. 4

Progress curve of fluorogenic substrate (Boc-EAR-MCA) hydrolysis by FXIa in the presence of PN2KPI-R15 K and the secondary plot. (A) Hydrolysis of Boc-EAR-MCA (275 µM) by FXIa (25 pM) in the presence of varying concentrations of PN2KPI-R15 K (0–120 nM) were monitored for 3,000 s. Inhibitor concentrations used are shown on the right, adjacent to the respective curve. (B) Secondary plot of kobs values versus PN2KPI-R15 K concentration. The kobs (s−1) values for each inhibitor concentration were calculated from the averaged values (± SD) of three assays each done in duplicate. All assays were background subtracted with substrate autolysis for the entire assay duration.