Table III.

Kinetic studies for inhibition of FXIa and trypsin by Kunitz inhibitors.

Enzyme	Inhibitor	Mechanism	Ki eq [nM]	k1 M−1 s−1	k–1 s−1	KI cal [nM]
FXIa	PN2KPI	1	0.81 ± 0.3	0.24 × 106	3.2 × 10−4	1.3
	PN2KPI-R15 K	1	11.3 ± 0.6	1.7 × 105	3.1 × 10−3	18.2
	BPTI	Competitive	627 ± 9.1			708 ± 81a
	BPTI-K15 R	1	4.1 ± 0.3	2.87 × 105	2.3 × 10−3	8.01
Trypsin	PN2KPI	1	0.03 ± 0.007	3.4 × 106	3.7 × 10−4	0.049
	PN2KPI-R15 K		0.047 ± 0.003
	BPTI	1	0.072 ± 0.001	4.4 × 105	3.2 × 10−5
	BPTI-K15 R		0.016 ± 0.002

^aCalculated from Michaelis–Menten plot represented in Fig. 5B.