Table III.
Enzyme | Inhibitor | Mechanism | Ki eq [nM] | k1 M−1 s−1 | k–1 s−1 | KI cal [nM] |
---|---|---|---|---|---|---|
FXIa | PN2KPI | 1 | 0.81 ± 0.3 | 0.24 × 106 | 3.2 × 10−4 | 1.3 |
PN2KPI-R15 K | 1 | 11.3 ± 0.6 | 1.7 × 105 | 3.1 × 10−3 | 18.2 | |
BPTI | Competitive | 627 ± 9.1 | 708 ± 81a | |||
BPTI-K15 R | 1 | 4.1 ± 0.3 | 2.87 × 105 | 2.3 × 10−3 | 8.01 | |
Trypsin | PN2KPI | 1 | 0.03 ± 0.007 | 3.4 × 106 | 3.7 × 10−4 | 0.049 |
PN2KPI-R15 K | 0.047 ± 0.003 | |||||
BPTI | 1 | 0.072 ± 0.001 | 4.4 × 105 | 3.2 × 10−5 | ||
BPTI-K15 R | 0.016 ± 0.002 |
aCalculated from Michaelis–Menten plot represented in Fig. 5B.