Skip to main content
. 2010 Oct 25;2(6):357–365. doi: 10.1093/jmcb/mjq031

Figure 2.

Figure 2

Regulation of endogenous RGS13 expression by the PKA activator cAMP in LAD2 mast cells. (A) Cells were stimulated with 2 mM 8-pCPT-cAMP for the time periods indicated. Cell lysates were prepared and analyzed by immunoblotting with anti-RGS13 antibody (upper panel) and anti-phosphoCREB(Ser133) to assess PKA activation (middle panel). Total RNA was isolated from a portion of the cells, followed by cDNA synthesis and analysis of RGS13 mRNA by real-time qPCR (lower panel). (B) Dose-response of RGS13 protein and mRNA levels after 3-h stimulation by cAMP. Cells were treated with a range of 8-pCPT-cAMP concentrations for 3 h followed by preparation of cell lysates and immunoblotting (upper panel) or extraction of RNA, cDNA synthesis and evaluation of RGS13 mRNA by qPCR (lower panel). RGS13 mRNA levels (bar graphs in both A and B) are presented as the fold control (mean ± SEM from three independent experiments;*P<0.001, one-way ANOVA).