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Inverse correlation between Stil and Chfr protein levels in vitro and in vivo. (A) Stil, Chfr and Plk1 protein levels in 1022M and 1022M+Stil cells (left panel), and in 3T3 cells expressing shRNA targeting Stil (right panel). (B) Immunoblot analysis of protein lysates from E9.5 embryos derived from mating between Stil+/− mice. K/O=Stil−/−, WT=Stil+/+, H=Stil+/−. α-Tubulin was used as loading control.
