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. 2011 Jan 18;124(4):565–577. doi: 10.1242/jcs.071274

Fig. 7.

Fig. 7.

Sarcomere assembly is delayed in slow-twitch muscle depleted of tnnt2c. (A–D) Mature sarcomeres can be seen in 24 hpf control embryos (A,C), but not in stage matched tnnt2c MO-injected embryos (B,D) stained for filamentous actin (A,B) and slow myosin (C,D). Scale bars: 25 μm, insets are 2× magnifications of corresponding panels. (E,F) Dysmorphology at the ultrastructural level is revealed by TEM images of slow muscle in 24 hpf control (E) and tnnt2c MO-injected embryos (F). Accumulations of Z line proteins (black arrowheads) and dispersed thick filaments (black arrow) can be found in the MO-injected embryos (F). Scale bars: 500 nm. (G,H) 30 hpf control embryos stained with an anti-titin antibody (G′,H′; green, T12), and phalloidin (G″,H″; red), show normal sarcomeres (G), whereas tnnt2c MO-injected embryos do not (H). Nuclei are counterstained with DAPI (G‴,H‴; blue). Single channels are shown in grey. The asterisk in H marks clustering nuclei in a lateral line rosette. Scale bars: 30 μm, insets are 2× magnifications of corresponding panels. (I,J) Slow-twitch muscle appeared normal at 5 dpf in control (I) and tnnt2c MO-injected (J) embryos. Scale bars: 50 μm.