Fig. 8.

OFD1 localizes to basal bodies in multiciliated epithelia. (A–C) Primary human nasal epithelial cells were grown to different stages of differentiation by transfer to an air–liquid interface before fixation and co-staining with antibodies against OFD1 (red) and γ-tubulin (green) (plus DNA, blue). Maximum intensity projections are shown in the upper panels. The lower panels show enlargements of the boxed areas through sequential z-sections. (D) Cells as in A–C were fixed co-stained with antibodies against OFD1 (red) and acetylated tubulin (green). (E) z-axis projections of the large panels in A–D. Cells in A are at day 10 post-transfer. Cells in B–D are at day 28 post-transfer.