Fig. 2.

BzATP specifically increased YO-PRO-1 uptake in AEC I. (A) Overnight-cultured AEC I and AEC II were incubated with 5 μM YO-PRO-1 in the absence (control) or the presence of 200 μM BzATP for 20 minutes, followed by incubation with 10 μM Nile Red. The cells were fixed and examined with a fluorescence microscope. Arrows point to AEC I. Scale bar, 40 μm. (B) AEC I and AEC II mixture or AEC II alone were incubated with 5 μM YO-PRO-1 for 5 minutes in the absence or presence of 100 nM BBG. The basal line was recorded with an excitation wavelength of 491 nm and an emission wavelength of 509 nm, and 100 μM BzATP was added at 4 minutes. The fluorescence intensities were corrected to the level of cells without the dye. Data are means±s.e.m. for three independent cell preparations.