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ChIP analysis showing interaction of RUNX1 with MYL9 promoter. (A) MYL9 upstream region showing 4 consensus sites for RUNX1. (B) ChIP analysis of endogenous RUNX1 binding in megakaryocytic HEL cells. Immunoprecipitation was performed using control IgG (lane 1) or anti-RUNX1 antibody (lane 2). Samples were analyzed by PCR using primers for MYL9 region −729/−542 nucleotides and GAPDH. Lanes 3 and 4 show PCR amplification of total input DNA and genomic DNA, respectively.
