Figure 3.

MDSC-IL-13 migrate to sites of allopriming in a GVHD setting. (A) Fluorescence-activated cell sorter analysis of pretransplantation MDSC-IL-13 was gated on Gr1⁺CD11b⁺ and analyzed for the surface expression of molecules associated with homing and recruitment. Gates are based on isotype controls. (B) Lethally irradiated BALB/c recipients were given 10⁷ BM cells plus 2 × 10⁶ T cells with 6 × 10⁶ eGFP transgenic MDSC-IL-13. Control mice were given BM and T cells only. On days 7 and 14, LN and spleen were harvested and imaged using macroscopic fluorescent imaging. (C) The absolute number of eGFP⁺ cells that had migrated to the LNs and spleen was determined using flow cytometry. (D) Spleen was harvested from day 7 transplanted mice, and flow cytometry was performed to determine whether MDSC-IL-13 retained Gr1⁺CD11b⁺ phenotype. Cells were gated on donor (GFP⁺) CD11b⁺ cells.