FIGURE 1:
APC/CAma1 is required for of Cdc20p destruction during meiosis. (A) Wild-type (RSY695), cdh1Δ (RSY1210), and ama1Δ (RSY853) strains harboring integrated Cdc20p-18myc were induced to enter meiosis and time points taken as indicated. Cdc20p-18myc levels were monitored by Western blot analysis. (B) The percent population of tetranucleated cells in the strains described in (A) are plotted. (C) Quantitation of the Cdc20p-18myc signal from (A) is plotted from the 9-h time point. The results shown are the averages from three separate experiments with error bars included. (D) Wild-type (RSY695) or mnd2Δ (KCY440) strains harboring integrated Cdc20p-18myc were induced to enter meiosis and time points taken as indicated. Western blot analysis of protein extracts was conducted to detect Cdc20p. The mnd2Δ strain remained mononucleated as previously described (Penkner et al., 2005). The blots were stripped and reprobed for Tub1p as a loading control. MI and MII indicate the approximate times of meiosis I (MI) and meiosis II (MII) as determined by DAPI analysis.