FIGURE 2:

Ama1p is required for normal meiotic divisions. (A) Wild-type (WT) (KCY224) and ama1Δ mutant (KCY225) harboring integrated Tub4p-GFP were induced to enter meiosis. Samples were fixed and analyzed by fluorescence microscopy at the time points indicated. Nuclear, spindle, and spindle pole body morphologies were determined by DAPI staining (top), indirect immunofluorescence of tubulin, and direct immunofluorescence of Tub4p-GFP, respectively. Magnification is 1000×. (B) Terminal meiotic arrest phenotype of ama1Δ mutant. WT and ama1Δ strains were induced to enter meiosis, and samples were taken after 24 h. Cells were scored as described in Materials and Methods. “Other” represents cells exhibiting irregular DAPI and tubulin staining. For all morphology quantitations presented, the SDs were ≤6% for all values. (C) Indirect immunofluorescence of tubulin and DAPI analysis of WT (RSY335) and ama1Δ (RSY562) cells after 24 h in sporulation medium. 1200× magnification.