Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Feb 1;22(3):315–326. doi: 10.1091/mbc.E10-04-0360

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Tan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — Identification of Cdc20p degrons. (A) Location of destruction boxes, KEN and GxEN motifs, in Cdc20p. Amino acid substitutions corresponding to the mutations generated are shown below the sequence. (B) Cdc20p deleted for either Db1 or GxEN is destroyed during meiosis with the same kinetics as wild type. Wild-type cells (RSY335) harboring the Cdc20p-18myc centromere plasmids indicated (see Supplemental Table S3 for details) were induced to enter meiosis and samples taken at the time points shown for Western analysis. The blots were stripped and reprobed for Tub1p as a loading control. (C) Cdc20p utilizes two degrons (Db1 and GxEN) for its destruction during meiosis. Cells were treated as described above except that Cdc20p harbored the mutations indicated.