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. 2011 Feb 1;22(3):327–341. doi: 10.1091/mbc.e10-09-0790

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© 2011 Deakin and Turner. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Hic-5 silencing promotes the adhesion-independent amoeboid phenotype in 3D ECM but does not prevent adhesion contact formation on 2D ECM. Immunofluorescence images of MDA-MB-231 cells treated with siRNA as stated for 69 h and then allowed to migrate (A) in 3D CDM or (B) on 2D FN-coated coverslips for 3 h. Cells were fixed and stained as indicated. All cells analyzed in four individual experiments, which were devoid of Hic-5 as assessed using the Hic-5-specific antibody, displayed the bleb-rich, adhesion-independent amoeboid phenotype in 3D ECM and robust adhesions on 2D FN.