FIGURE 3:
lst-4 and snx-1 are both required for the efficient incorporation of lysosomes and early endosomes to phagosomes. (A–D) DIC and epifluorecent images of adult hermaphrodite gonads expressing Pced-1ctns-1::gfp. Arrows indicate phagosomes containing germ cell corpses. Arrowheads in (B) indicate nascent phagosomes not yet labeled with CTNS-1::GFP. γ-Irradiation (IR) was used in (B) to increase the number of apoptotic cells in wild-type animals. Scale bars, 20 μm. (E, G, and I) Data are presented as mean ± SD. Fifteen animals were scored for each sample. Independent Student’s t-test was used to calculate P values between wild-type and mutant animals. NS: not significant. *, P < 0.005; **, P < 0.001. (E) The percentage of CTNS-1::GFP(+) phagosomes in adult hermaphrodite gonads at 48 h post-L4 stage. (F) Epifluorecent images of 1.5-fold stage embryos expressing Pced-1ctns-1::gfp. Arrowheads indicate CTNS-1::GFP particles in hypodermal cells. An arrow indicates CTNS-1::GFP(+) phagosome. Scale bars: 10 μm. (G) The percentage of CTNS-1::GFP(+) phagosomes in 1.5-fold stage embryos. (H) DIC (a and c) and epifluorecent (b and d) images of 1.5-fold stage embryos expressing Pced-1hgrs-1::gfp. Arrows indicate phagosomes. Scale bars: 10 μm. (I) The percentage of HGRS-1::GFP(+) phagosomes in 1.5-fold stage embryos.