FIGURE 8:
The localization patterns of LST-4 and SNX-1 on phagosomes are primarily controlled by the ced-1 pathway. (A–D) and (F–I) Time-lapse images of indicated GFP reporters on maturing phagosomes in wild-type (A and F) or ced mutant embryos (B–D and G–I) expressing Pced-1lst-4(d)::gfp or Pced-1 snx-1::gfp, respectively. Arrows indicated GFP signals on phagosomes. “0 min” represents the time point when the phagosome is just sealed. Scale bars, 2 μm. (E and J) Quantification of the different localization patterns of LST-4(d)::GFP (E) and SNX-1::GFP (J) on phagosomes in wild-type and ced-1 or ced-5 mutant embryos. Types of localization patterns are defined as follows: “normal recruitment,” GFP(+) circles were detected on phagosomes within 8 min after the engulfment was completed; “delayed recruitment,” GFP(+) circles were detected on the phagosomes at least 10 min after the engulfment was completed; “no recruitment,” GFP signals were not detected on the phagosomes for a period > 50 min starting from the completion of engulfment; “short association,” the GFP signal was recruited to phagosomes at the normal time point, yet quickly disappeared from phagosomal surfaces.