TABLE 1:
A candidate genetic screen for PtdIns(3)P effectors acting in the removal of apoptotic cells. The numbers of germ cell corpses were scored in the gonads of adult hermaphrodites at 48 h post-L4 stage. Data are presented as mean ± SD. n, number of animals scored. The names of C. elegans genes and deletion alleles were used according to the wormbase (http://www.wormbase.org). The name of Homo sapiens was used according to the unigene database of NCBI (http://www.ncbi.nlm.nih.gov).
| Genotype | Number of germ cell corpses | Mammalian homologue |
|---|---|---|
| wild-type | 2.8 ± 1.1 (n = 15) | N/A |
| FYVE domain-containing proteins | ||
| C26H9A.2(ok912) | 8.3 ± 3.5 (n = 15) | WDFY3 |
| mtm-6(ok330) | 5.5 ± 2.3 (n = 13) | MTMR6 |
| tag-77(tm702) | 1.9 ± 1.6 (n = 10) | FGD6 |
| aka-1(tm389) | 4.7 ± 1.6 (n = 15) | ZFYVE9 |
| eea-1(tm933) | 1.5 ± 2.0 (n = 15) | EEA1 |
| exc-5(rh232) | 1.2 ± 1.7 (n = 10) | FGD2 |
| rabs-5(ok1513) | 1.1 ± 1.2 (n = 10) | Rabenosyn-5 |
| rabn-5(tm1555) | 1.8 ± 1.2 (n = 10) | Rabaptin-5 |
| F22G12.4(tm3221) | 2.4 ± 1.2 (n = 15) | ANKFY1 |
| wdfy-2(tm3806) | 2.6 ± 2.4 (n = 15) | WDFY2 |
| lst-2(RNAi) | 4.3 ± 2.2 (n = 17) | ZFYVE28 |
| ZK632.12(RNAi) | 3.2 ± 1.6 (n = 10) | Phafin 2 |
| hgrs-1(RNAi) | 4.4 ± 1.4 (n = 11) | Hrs |
| mtm-3(RNAi) | 4.1 ± 2.7 (n = 20) | MTMR3 |
| R11D1.10(RNAi) | 2.8 ± 2.4 (n = 15) | ZFYVE19 |
| ppk-3(RNAi) | 3.7 ± 2.5 (n = 15) | PIKfyve |
| PX domain-containing proteins | ||
| pld-1(ok2222) | 1.9 ± 1.8 (n = 15) | PLD 1 |
| snx-1(tm847) | 19.1 ± 3.5 (n = 15) | SNX 1/2 |
| F13E9.1(tm1886) | 1.8 ± 1.7 (n = 10) | Nischarin |
| Y116A8C.26(tm2404) | 1.6 ± 1.3 (n = 10) | SNX 13 |
| snx-3(tm1595) | 2.7 ± 1.8 (n = 17) | SNX 3/12 |
| lst-4(tm2423) | 60.7 ± 7.8 (n = 15) | SNX 9/18/33 |
| F39B1.1(tm3171) | 38.7 ± 6.0 (n = 15) | PI3KC2α |
| F17H10.3(tm3643) | 2.3 ± 1.3 (n = 15) | SNX 17 |
| rskd-1(tm4031) | 1.7 ± 0.7 (n = 15) | RPS6KL1 |
| snx-6(tm3790) | 13.3 ± 4.0 (n = 15) | SNX 5/6 |
| Y48E1B.14(RNAi) | 4.3 ± 2.5 (n = 15) | SNX 14 |
| F25H2.2(RNAi) | 3.9 ± 1.9 (n = 16) | SNX 27 |