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. 2011 Feb 1;22(3):421–436. doi: 10.1091/mbc.e10-10-0807

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© 2011 Won et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Characterization of aPSC in culture. A morphological characterization is shown in (A). (Aa) Typical DIC images of aPSC at day 7 in culture. Prominent staining with α-SMA (Ab), vimentin (Ac), and desmin (Ad). (B–E) Representative Ca²⁺ imaging data from aPSC at day 7 in culture. Increases in [Ca²⁺]_i were evoked by angiotensin (Ba) and bradykinin (Bb) but not KCl (Bc). (C) Cells responded to ATP. (D) Comparison of the concentration vs. response relationship for the ATP-evoked change in peak height in aPSC vs. qPSC. In contrast to qPSC, aPSC did not increase Ca²⁺ when challenged with CCh (Ea), but thrombin (Eb), trypsin (Ec), and PDGF (Ed) evoked robust elevations in [Ca²⁺]_i.