FIGURE 5:

Characterization of PAR Ca²⁺ signaling in aPSC in culture. (A) Ca²⁺ elevations could be evoked in aPSC following exposure to peptide PAR-1 peptide ligand (SFLLRN-NH₂) (Aa) and PAR-2 peptide ligand (SLIGRL-NH₂) (Ab) but not PAR-3 (SFNNGGP-NH₂) (Ac) or PAR-4 ligand (AYPGKF-NH₂) (Ad). (B) Concentration vs. response relationship for the peak Ca²⁺ response evoked by PAR-1 ligand. (C) Increasing PAR-1 ligand results in progressive recruitment of cells to increase Ca²⁺ (increase defined as > 0.02 ratio units change from basal). (D) Mean data showing the change in latency and peak height of the Ca²⁺ signal initiated by increasing [PAR-1] ligand.