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. 2011 Jan 31;6(1):e14636. doi: 10.1371/journal.pone.0014636

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Gilbert et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Line A and B chicks were inoculated at day 15 with E. acervulina (EA), E. maxima (EM) or E. tenella (ET), or the vehicle (control; C). Blood was collected and lesion scoring was performed on the small intestine. Serum samples were depleted of IgG and albumin and composited. Two-dimensional electrophoresis was perfomed and gels were stained with Flamingo stain. Protein spot density was analyzed and spots showing a significant effect of coccidia strain and/or broiler genetic line at P<0.05−0.01 (250 spots), P<0.01−0.001 (248 spots), and P<0.001 (314 spots) were excised and analyzed by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry.