Fig. 3.

Modulation of the expression of Tep genes in adults after septic injury. a, b Drosophila Teps are located on the left arm of the second chromosome. a Tep2 (CG7052) and Tep3 (CG7068) map to cytogenetic position 28C1. Tep2 and Tep3 are 1.5 kb apart. The modified XP transposable element XP11521 is inserted 22 bp upstream of the transcription-starting site of Tep2, whereas the XP03976 element is inserted 121 bp downstream of the transcription- starting site of Tep3. We have generated a double mutant for Tep2 and Tep3, Tep2, 3, by FLP-mediated recombination between the FRT elements carried on the XP elements. b Tep4 (CG10363) maps to cytogenetic position 37F1. Transposable elements inserted in this region are shown. We have used EY04656 as a mutant for Tep4 as this modified P element is inserted in the initiation codon of the Tep4 gene. In the following experiments, we used an RNAi transgenic line to knock down Tep1 (CG18096). c-f Steadystate transcript levels of D. melanogaster Tep genes were measured by quantitative RT-PCR before and after infection with a mix of E. coli and M. luteus. These experiments are representative of at least two independent experiments. 0 h = Non-infected flies; 3 h/6 h/48 h = flies 3/6/48 h after infection; WT = wild type. Flies were frozen for experiments 3, 6, and 48 h after infection. Gene expression was normalized against rp49 gene expression and the results are expressed as percentage of maximal expression: 6 (Tep1; c) and 3 h after infection (Tep2 and Tep4; d, e). Expression of a Tep6 transgene under the control of a heat shock promoter using the UAS-GAL4 system (hsp>Tep6) after heat shock (f). ∗ p<0.05. c The induction of Tep1 is decreased by the expression of an RNAi transgene targeting specifically this gene.